Research Fellow (Chemistry)

Interested applicants are invited to apply directly at the NUS Career Portal. Please note your application will only be processed if you apply via NUS Career Portal.

NUS Career Portal link - https://careers.nus.edu.sg/job/Research-Fellow-%28Chemistry%29/34137-en_GB/?st=30E1EEE12BEFE4FB4AA072FA5DC2AF1E10B07217

We regret that only shortlisted candidates will be notified.

Job Description

The successful candidate will work with Asst Prof WEI Jiangbo on physiological functions of RNA modifications and RNA binding proteins under a project on "Circulating Cell-Free RNA modification (cfRNA-mod) for MASH Diagnosis/Monitoring".

The main responsibilities of the position include:
1. Establish the model system to study context-dependent crosstalk between epitranscriptome and epigenome in the lab.
2. Carry out biochemistry and cell biology-related experiments and bioinformatics work for associated omics.
3. Facilitate grant application.
4. Aid and support to junior PhD students in their research projects.
5. Lead a small group of PhD working on the physiological roles of RNA modifications and RBP in development.
6. Report duty to PI.

Qualifications / Discipline:
• The candidate should hold a PhD degree or have submitted PhD thesis, in Biochemistry and Molecular Biology, or related disciplines.

Skills:
• Proficient in a wide range of bench techniques relevant to biochemistry, molecular biology, chemical biology, genetics, and cell biology.
• Skills include, but are not limited to: chemical synthesis, nucleic acid extraction and purification, molecular cloning, plasmid preparation, PCR and RT-qPCR, site-directed mutagenesis, restriction enzyme digestion, ligation, gel electrophoresis (agarose and SDS-PAGE), immunoblotting (Western blot), immunoprecipitation, ELISA, cell culture (bacterial, yeast, and mammalian), cell transfection and transduction, CRISPR-Cas9 genome editing, Sanger and next-generation sequencing (NGS) library preparation, immunofluorescence and confocal microscopy, flow cytometry, protein expression and purification, enzyme assays, and spectrophotometric analysis.
• Has extensive experience in model system that suitable to study the role of RNA modifications and RBPs in development.

Experience:
• Have published (or at least submitted) peer-reviewed papers as the first author in high-impact journals.