Research Associate

We are seeking a highly motivated PhD Research Associate to join our R&D activities for CRISPR-Cas-based detection projects.

The successful candidate will work on engineering Cas-enzymes, assay development, optimization, and validation of CRISPR-Cas-based molecular diagnostics.

This role requires hands-on experience in protein engineering, molecular biology and assay development, excellent problem-solving skills, and a strong interest in innovative research and technology development.

Key Responsibilities:

• Engineering Cas enzyme
• Design and optimize CRISPR-Cas-based detection assays for various targets
• Conduct molecular biology experiments, including nucleic acid extraction, amplification (PCR/RPA), and CRISPR-mediated detection
• Perform fluorescence and lateral flow-based readouts for assay validation
• Troubleshoot and improve assay sensitivity, specificity and reproducibility
• Enhance enzyme performance through directed evolution
• Analyse experimental data, prepare reports, and present findings to the team
• Collaborate with multidisciplinary teams, including bioinformatics and engineering groups
• Maintain accurate lab records and ensure compliance with safety regulations
• Contribute to scientific publications and patent filings as needed

Qualifications & Skills:

Essential

• PhD in Molecular Biology, Biotechnology, Biochemistry, or a related field
• Strong hands-on experience in molecular biology techniques, including protein expression and purification, and RT-PCR
• Experience in protein/enzyme engineering
• Knowledge of CRISPR-Cas technologies and their applications, particularly Cas12 and Cas13
• Strong analytical and problem-solving skills
• Ability to work independently and collaboratively within a team

Highly Desirable

• Experience with isothermal amplification methods, such as RPA or LAMP
• Experience in DNA/RNA detection and assay development
• Familiarity with fluorescence-based and/or lateral flow detection methods
• Knowledge of bioinformatics tools for CRISPR guide design

General Skills

• Excellent organisational skills and attention to detail
• Strong verbal and written communication skills